• 产品名称Anti-SIK3抗体
    参阅全部 SIK3 一抗
  • 描述
    兔多克隆抗体to SIK3
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide mapping to a region between residue 900 and 950 of human SIK3 using the numbering given in entry NP_079440.2

  • 阳性对照
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab88495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP Use at 2-5 µg/mg of lysate.


  • 相关性Activated by phosphorylation on Thr 163 by STK11 in complex with STE20 related adapter alpha (STRAD alpha) pseudo kinase and CAB39. Binds to and is activated by YWHAZ when phosphorylated on Thr 163.
  • 细胞定位Cytoplasm
  • 数据库链接
  • 别名
    • FLJ12240 antibody
    • KIAA0999 antibody
    • L19 antibody
    • QSK antibody
    • Salt inducible kinase 3 antibody
    • serine/threonine-protein kinase QSK antibody
    • Serine/threonine-protein kinase SIK3 antibody
    • SIK 3 antibody
    • SIK family kinase 3 antibody
    • SIK3_HUMAN antibody
    see all

Anti-SIK3 antibody 图像

  • All lanes : Anti-SIK3 antibody (ab88495) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg

    Predicted band size : 140 kDa

    Exposure time : 30 seconds
  • 3ug ab88495 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88495 at 1ug/ml.
    Detection: chemoluminescence with exposure time of 3 minutes.
  • ICC/IF image of ab88495 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SIK3 antibody (ab88495)参考文献

ab88495 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, we do not have the corresponding blocking peptide available to buy in the catalogue.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 50 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Jul 20 2012