The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
RNA polymerase sigma 32 factor antibody
rpo H antibody
Western blot - Sigma 32 antibody (ab26890)
Lane 1 : Pre-bleed at 1/2000 dilution Lane 2 : Anti-serum at 1/2000 dilution
Lane 1 : Antigen Lane 2 : Antigen
Lysates/proteins at 4 µg per lane.
Secondary anti-rabbit IgG-HRP Developed using the ECL technique