Anti-Sigma 32抗体(ab26890)

概述

  • 产品名称
    Anti-Sigma 32抗体
  • 描述
    兔多克隆抗体to Sigma 32
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    Reacts with Serratia marcescens. Not yet tested in other species.
  • 免疫原

    Full length Sigma 32 protein (Serratia marcescens)

性能

应用

Our Abpromise guarantee covers the use of ab26890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

靶标

  • 相关性
    When cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
  • 别名
    • RNA polymerase sigma 32 factor antibody
    • rpo H antibody
    • rpoH antibody

图片

  • Lane 1 : Pre-bleed at 1/2000 dilution
    Lane 2 : Anti-serum at 1/2000 dilution

    Lane 1 : Antigen
    Lane 2 : Antigen

    Lysates/proteins at 4 µg per lane.

    Secondary
    anti-rabbit IgG-HRP
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab26890 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
10.9912 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Escherichia coli Cell lysate - whole cell (no specification)
Specification
no specification
Treatment
100 ´M Nalidixic Acid for 18 hours
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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