概述
- 产品名称Anti-SIGLEC8抗体
参阅全部 SIGLEC8 一抗 - 描述兔多克隆抗体to SIGLEC8
- 经测试应用适用于: ELISA, WB, IHC-Pmore details
- 种属反应性与反应: Mouse, Human
- 免疫原
Synthetic peptide (10-30 amino acids in length) conjugated to KLH, selected from within amino acids 413-431 of Human SIGLEC8.
- 阳性对照
- HL60 cell lysate, Mouse liver tissue lysate, Human breast carcinoma tissue.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - 纯度Protein G purified
- 纯化说明This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab38578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/1000. | |
| WB | 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
- 功能Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
- 组织特异性Expressed specifically on eosinophils.
- 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. - 结构域Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
- 细胞定位Membrane.
- Information by UniProt
-
数据库链接
- Entrez Gene: 27181 Human
- Omim: 605639 Human
- SwissProt: Q9NYZ4 Human
- Unigene: 447899 Human
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别名
- CD329 antigen antibody
- CDw329 antibody
- SAF 2 antibody
see all
Anti-SIGLEC8 antibody 图像
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Western blot - SIGLEC8 antibody (ab38578)All lanes : Anti-SIGLEC8 antibody (ab38578) at 1/100 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : HL60 cell lysate
Predicted band size : 54 kDa
Observed band size : 54 kDa
Additional bands at : 14 kDa,37 kDa,75 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SIGLEC8 antibody (ab38578)ab38578 staining SIGLEC8 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab38578 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Anti-SIGLEC8 antibody (ab38578)参考文献
ab38578 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"