Anti-SIGLEC8抗体(ab38578)

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概述

  • 产品名称
    Anti-SIGLEC8抗体
    参阅全部 SIGLEC8 一抗
  • 描述
    兔多克隆抗体to SIGLEC8
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, selected from within amino acids 413-431 of Human SIGLEC8.

  • 阳性对照
    • HL60 cell lysate, Mouse liver tissue lysate, Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab38578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • 组织特异性
    Expressed specifically on eosinophils.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CD329 antigen antibody
      • CDw329 antibody
      • SAF 2 antibody
      • SAF-2 antibody
      • SAF2 antibody
      • Sialic acid binding Ig like lectin 8 antibody
      • Sialic acid-binding Ig-like lectin 8 antibody
      • Sialoadhesin family member 2 antibody
      • SIGL8_HUMAN antibody
      • Siglec 8 antibody
      • Siglec-8 antibody
      • SIGLEC8 antibody
      • SIGLEC8L antibody
      see all

    图片

    • Western blot - Anti-SIGLEC8 antibody (ab38578)
      Western blot - Anti-SIGLEC8 antibody (ab38578)
      All lanes : Anti-SIGLEC8 antibody (ab38578) at 1/100 dilution

      Lane 1 : Mouse liver tissue lysate
      Lane 2 : HL60 cell lysate

      Predicted band size: 54 kDa
      Observed band size: 54 kDa
      Additional bands at: 14 kDa, 37 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC8 antibody (ab38578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIGLEC8 antibody (ab38578)
      ab38578 staining SIGLEC8 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab38578 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    文献

    ab38578 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab38578？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1 Review or Q&A

    10
    Votes: Highest First

    Hallo Team

    Ein Kunde fragt, ob der Artikel ab38578 auch für IHC Parafin geeignet ist?

    Habt Ihr Bilder von Testresultate welche schon mit IHC getestet wurden?

    Danke für Eure Antwort.

    Vielen Dank für Ihre Anfrage.

    In der Tat haben wir den Anti-SIGLEC8-Antikörper ab38578 in der IHC an Paraffin-eingebetteten Schnitten (Mammakarzinom, Hepatokarzinom) getestet und können garantieren, dass er in dieser Anwendung funktioniert, ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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