概述

  • 产品名称Anti-SIGLEC5抗体[1A5]
    参阅全部 SIGLEC5 一抗
  • 描述
    小鼠单克隆抗体[1A5] to SIGLEC5
  • 特异性This antibody cross reacts with SEGLEC14.
  • 经测试应用适用于: IHC-Fr, ELISA, Flow Cyt, ICC/IF, IP, Neutralising, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Siglec-5-Fc protein, containing entire extracellular region of siglec-5 fused with the Fc region of human IgG1.

  • 阳性对照
    • Siglec 5 transfected CHO cells.

性能

应用

Our Abpromise guarantee covers the use of ab97426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.

靶标

  • 功能Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • 组织特异性Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
  • 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD170 antibody
    • CD170 antigen antibody
    • CD33 antigen-like 2 antibody
    • CD33L2 antibody
    • mSiglec-F antibody
    • OB-binding protein 2 antibody
    • OB-BP2 antibody
    • OBBP2 antibody
    • Obesity-binding protein 2 antibody
    • Sialic acid binding Ig-like lectin 5 antibody
    • Sialic acid binding immunoglobulin-like lectin 5 antibody
    • Sialic acid-binding Ig-like lectin 5 antibody
    • Sialic acid-binding Ig-like lectin F antibody
    • SIGL5_HUMAN antibody
    • Siglec-5 antibody
    • Siglec-F variant antibody
    • Siglec5 antibody
    • Siglecf antibody
    see all

Anti-SIGLEC5 antibody [1A5] 图像

  • Overlay histogram showing THP1 cells stained with ab97426 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97426, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-SIGLEC5 antibody [1A5] (ab97426)参考文献

This product has been referenced in:
  • Cornish AL  et al. Characterization of siglec-5, a novel glycoprotein expressed on myeloid cells related to CD33. Blood 92:2123-32 (1998). Read more (PubMed: 9731071) »

See 1 Publication for this product

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Thank you for your inquiry.
Unfortunately, the immunogen of ab97426 only shows 50% homology with mouse and we therefore would not expect a cross reaction.
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