概述

  • 产品名称
  • 描述
    山羊多克隆抗体to SIDT1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    RFQRKSIDGSFGSN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 334-347 of Human SIDT1 (NP_060169.2).

  • 阳性对照
    • Human cerebellum lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab123808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).

靶标

Anti-SIDT1 antibody 图像

  • All lanes : Anti-SIDT1 antibody (ab123808) at 0.300000011921 µg/ml

    Lane 1 : Human spleen lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Mouse thymus lysate
    Lane 5 : Rat thymus lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 94 kDa
  • Anti-SIDT1 antibody (ab123808) at 0.3 µg/ml (incubation time: 1 hour) + Human cerebellum lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-SIDT1 antibody (ab123808)参考文献

ab123808 has not yet been referenced specifically in any publications.

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