Anti-Sialyl Tn抗体[STn 219] (ab115957)


  • 产品名称Anti-Sialyl Tn抗体[STn 219]
    参阅全部 Sialyl Tn 一抗
  • 描述
    小鼠单克隆抗体[STn 219] to Sialyl Tn
  • 经测试应用适用于: Flow Cyt, IHC-P, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Sheep, Human
  • 免疫原

    Ovine submaxillary mucin (OSM).

  • 表位NeuAc a GalNacOSer/Thr.
  • 阳性对照
    • Human gastrointestinal tumor, prostate and ovary carcinoma tissues.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 15th June 2017.  The following lots are from ascites and still in stock as of 15th June 2017 (GR271829-1, GR293422-1, GR247695-1, GR293422-2, GR293422-1 ).  Lot numbers higher than GR293422-2 will be from tissue culture supernatant.  Please note that the dilutions may need to be adjusted accordingly.



Our Abpromise guarantee covers the use of ab115957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

Use at an assay dependent concentration


  • 相关性Sialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains
  • 别名
    • CD175s antibody
    • Sialosyl Tn antibody
    • sTn antibody

Anti-Sialyl Tn antibody [STn 219] 图像

  • Overlay histogram showing MCF7 cells stained with ab115957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115957, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Sialyl Tn antibody [STn 219] (ab115957)参考文献

ab115957 has not yet been referenced specifically in any publications.

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