Anti-Sialoadhesin抗体(ab94715)

概述

  • 产品名称Anti-Sialoadhesin抗体
    参阅全部 Sialoadhesin 一抗
  • 描述
    兔多克隆抗体to Sialoadhesin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Sialoadhesin.

  • 阳性对照
    • This antibody gave a positive signal in Human Spleen Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab94715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 183 kDa).

靶标

  • 功能Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • 组织特异性Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • 序列相似性Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Anti-Sialoadhesin antibody 图像

  • Anti-Sialoadhesin antibody (ab94715) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 183 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

Anti-Sialoadhesin antibody (ab94715)参考文献

ab94715 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Pig Cell lysate - other (Alveolar macrophages)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 20000 cells
Specification Alveolar macrophages
Blocking step PBS/0.1% Tween 20/5% skimmed milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

David Winder

Verified customer

提交于 Aug 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"