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Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in a variety of biological samples. The detection is based in an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).
The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
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Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.
|Sialic Acid Assay Buffer||WM||1 x 25ml|
|Sialic Acid Converting Enzyme (lyophilized)||Purple||1 vial|
|Sialic Acid Development Mix (lyophilized)||Green||1 vial|
|Sialic Acid Probe (in DMSO)||Red||1 x 200µl|
|Sialic Acid Standard (10 umol) (lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"