Sialic Acid (NANA) Assay试剂盒(ab83375)

概述

  • 产品名称Sialic Acid (NANA) Assay试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    1 µM
  • 范围
    0.1 µM - 10 µM
  • 检测时间
    0h 40m
  • 产品概述

    Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in a variety of biological samples. The detection is based in an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).

    The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been show recently that sialic acid level may associate with developmental and pathological stages.
  • 别名
    • N acetylneuraminic acid
    • NANA

应用

Our Abpromise guarantee covers the use of ab83375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Sialic Acid (NANA) Assay Kit 图像

  • Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated

  • Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375.

实验方案

Sialic Acid (NANA) Assay Kit (ab83375)参考文献

ab83375 has not yet been referenced specifically in any publications.

Product Wall

I am pleased to let you know that the sialic acid standards are now available separately under the product number ab170150.

To our knowledge only mammalian samples have been tested with this kit and we are not sure whether crude plant extracts would be compatible. The kit is designed to detect free sialic acid.


We have used this kit only with mammalian samples, therefore we cannot comment on how well it would work with plant samples. Since the principle is the same, this kit should work with samples from multiple sources including plants. However, you ma...

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Thank you for contacting us.

Yes, the SA will get out into the filtrate. That is the whole purpose of filtering this. The SA will get out into the filtrate and the higher MW interfering agents will be stuck at the top of the filter.
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Thank you for contacting us.

You could try the following protocol for gycoprotein, glycolipid or glycan samples:

Sample is dissolved in a final concentration of 2 M HOAc and heated to 80°C for 3 hours to release sialic acids. Th...

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Thank you very much for your inquiry about the ab83375.

This is a very good question.

I am sorry however that this kit will not be suitable for your needs. Indeed, as you mention, for measuring the bound sialic acid, the sample must...

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Thank you for contacting us. Could you please describe more fully the problems your customer is encountering? Does she observe the expected results when using the Sialic acid standard provided with the kit? In the protocol provided with the kit the...

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Thank your for your interest in this product. In answer to your questions: 1. Her sample is cell culture supernatant. Can it be used without any preprocessing? Cell culture supernatant should not require pre-processing in order to utilise the k...

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Thank you for your inquiry. I can confirm that the sample types tested for this kit are cell and tissue culture supernatants, urine, plasma & serum, and other biological fluids. This does mean media, and not lysate. I f your customer is plan...

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Thank you for your enquiry. I can confirm that the sample volume to be used with ab83375 Sialic Acid (NANA) Assay Kit will very much depend on the levels of this metabolite in the sample. It generally has to be in the linear range of the assay ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"