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Our Abpromise guarantee covers the use of ab2237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 17189520
1:300 donkey anti-goat was used for secondary antibody.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28, 30 kDa (predicted molecular weight: 31, 35 kDa).Can be blocked with Human SIAH1 peptide (ab22876).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|