概述

  • 产品名称Anti-SIAE抗体
  • 描述
    小鼠多克隆抗体to SIAE
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human SIAE protein (NP_733746.1)

  • 阳性对照
    • Human colon tissue lysate. SIAE transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

靶标

  • 相关性Sialic acids are acidic 9-carbon sugars typically found at the non-reducing end of sugar chains. They are frequently modified by 9-O-acetylation and this modification is removed by sialic acid acetylesterases. SIAE catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively). It is widely expressed with high expression in the testis, prostate, and colon. There are three named isoforms.
  • 细胞定位Lysosome
  • 数据库链接
  • 别名
    • CSE C antibody
    • CSEC antibody
    • Cytosolic sialic acid 9 O acetylesterase homolog antibody
    • H Lse antibody
    • HLse antibody
    • LSE antibody
    • MGC87009 antibody
    • Sialate O acetylesterase antibody
    • Sialic acid acetylesterase antibody
    • Sialic acid specific 9 O acetylesterase antibody
    • Sialic acid specific acetylesterase II antibody
    • YSG2 antibody
    • Ysg2 homolog antibody
    see all

Anti-SIAE antibody 图像

  • Anti-SIAE antibody (ab69300) at 1/500 dilution + human colon tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 21 kDa,26 kDa,32 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SIAE antibody (ab69300) at 1/500 dilution

    Lane 1 : SIAE transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

Anti-SIAE antibody (ab69300)参考文献

ab69300 has not yet been referenced specifically in any publications.

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