概述

  • 产品名称Anti-SI抗体
    参阅全部 SI 一抗
  • 描述
    兔多克隆抗体to SI
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide designed within residues: NSVLFTTQNQ TPNRFRFKIT DPNNRRYEVP HQYVKEFTGP TVSDTLYDVK, corresponding to N terminal amino acids 144-193 of Human SI (NP_001032)

  • 阳性对照
    • Fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab84977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 209 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Plays an important role in the final stage of carbohydrate digestion.
  • 组织特异性Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
  • 疾病相关Defects in SI are the cause of congenital sucrase-isomaltase deficiency (CSID) [MIM:222900]; also known as disaccharide intolerance I. CSID is an autosomal recessive intestinal disorder that is clinically characterized by fermentative diarrhea, abdominal pain, and cramps upon ingestion of sugar. The symptoms are the consequence of absent or drastically reduced enzymatic activities of sucrase and isomaltase. The prevalence of CSID is 0.02 % in individuals of European descent and appears to be much higher in Greenland, Alaskan, and Canadian native people. CSID arises due to post-translational perturbations in the intracellular transport, polarized sorting, aberrant processing, and defective function of SI.
  • 序列相似性Belongs to the glycosyl hydrolase 31 family.
    Contains 2 P-type (trefoil) domains.
  • 翻译后修饰The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen.
    Sulfated.
  • 细胞定位Apical cell membrane. Brush border.
  • Information by UniProt
  • 数据库链接
  • 形式Sucrase-isomaltase is composed of 2 polypeptide chains of similar size linked together by noncovalent bonds. The polypeptide chains show a high degree of overlapping substrate specificity and are synthesized as 1 long polypeptide chain which, by means of pancreatic proteases, is later split into the 2 subunits.
  • 别名
    • Isomaltase antibody
    • MGC131621 antibody
    • MGC131622 antibody
    • Oligosaccharide alpha 1, 6 glucosidase antibody
    • SI antibody
    • sucrase isomaltase alpha glucosidase antibody
    • sucrase isomaltase antibody
    • Sucrase-isomaltase, intestinal antibody
    • SUIS_HUMAN antibody
    see all

Anti-SI antibody 图像

  • Anti-SI antibody (ab84977) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 209 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

Anti-SI antibody (ab84977)参考文献

ab84977 has not yet been referenced specifically in any publications.

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