概述

  • 产品名称Anti-Shugoshin抗体
    参阅全部 Shugoshin 一抗
  • 描述
    兔多克隆抗体to Shugoshin
  • 特异性There are 7 isoforms of Shugoshin with the following sizes: 64kDa, 58kDa, 34kDa, 32kD, 30kDa, 28kDa, and 24kDa. The immunogen used for this antibody is present in all isoforms. This antibody interacts with several proteins in the unsynchronized cell lines tested, with the most prominent bands in the range of 30-34kDa. The cell samples may need to be synchronized to detect the larger isoforms (see PLoS Biol. 2005 Mar;3(3):e86). These bands are competed away by addition of the immunizing peptide.
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Shugoshin.

    (Peptide available as ab23438.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa Whole Cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab21633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ICC/IF 1/1000. PubMed: 18772885
IHC-P 1/350. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
  • 组织特异性Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.
  • 序列相似性Belongs to the shugoshin family.
  • 发展阶段Appears in prophase cells and remains present until metaphase. Strongly decreases at the onset of anaphase and completely disapears at telophase. Not present in interphase cells (at protein level).
  • 结构域The KEN box and D-box 3 are required for its ubiquitination and degradation.
  • 翻译后修饰Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
    Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB.
  • 细胞定位Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > centrosome. Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Co-localizes with NEK2 and SS18L1 at the kinetochore. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis. Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent. Isoform 3 does not localize to kinetochores during any stages of the cell cycle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSgo 1 antibody
    • hSgo1 antibody
    • NY BR 85 antibody
    • Serologically defined breast cancer antigen NY BR 85 antibody
    • Serologically defined breast cancer antigen NY-BR-85 antibody
    • SGO 1 antibody
    • SGO antibody
    • SGO L1 antibody
    • SGO1 antibody
    • SGOL 1 antibody
    • SGOL1 antibody
    • SGOL1_HUMAN antibody
    • Shugoshin 1AB protein antibody
    • Shugoshin 1CD protein antibody
    • Shugoshin 1EFprotein antibody
    • Shugoshin 1GH protein antibody
    • Shugoshin 1KL protein antibody
    • Shugoshin like 1 (S. pombe) antibody
    • Shugoshin like 1 antibody
    • Shugoshin-like 1 antibody
    see all

Anti-Shugoshin antibody 图像

  • Anti-Shugoshin antibody (ab21633) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 34 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Image courtesy of Human Protein Atlas

    ab21633 staining Shugoshin in Human hippocampus. Paraffin embedded human hippocampus tissue was incubated with ab21633 (1/350 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab21633 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Anti-Shugoshin antibody (ab21633)参考文献

This product has been referenced in:
  • Murakami-Tonami Y  et al. SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. Sci Rep 6:31615 (2016). Mouse . Read more (PubMed: 27539729) »
  • Lee MY  et al. Differential expression of centrosome regulators in Her2+ breast cancer cells versus non-tumorigenic MCF10A cells. Cell Div 9:3 (2014). Read more (PubMed: 25278993) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% Trition
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Chelsea Backer

Verified customer

提交于 Oct 24 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jun 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"