Anti-SHPRH抗体(ab80129)

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概述

  • 产品名称Anti-SHPRH抗体
    参阅全部 SHPRH 一抗
  • 描述
    兔多克隆抗体to SHPRH
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human SHPRH.

    参阅Abcam的专有抗源政策

    (Peptide available as ab89391.)

  • 阳性对照
    • This antibody gave a positive signal in SHSY-5Y whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).

靶标

  • 功能E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
  • 组织特异性Broadly expressed.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 结构域The RING finger mediates E3 ubiquitin ligase activity.
  • Information by UniProt
  • 数据库链接
    • 别名
      • bA545I5.2 antibody
      • E3 ubiquitin-protein ligase SHPRH antibody
      • FLJ27258 antibody
      • FLJ37625 antibody
      • FLJ45012 antibody
      • FLJ90837 antibody
      • histone-linker antibody
      • KIAA2023 antibody
      • MGC134886 antibody
      • PHD and RING finger domain-containing helicase antibody
      • Shprh antibody
      • SHPRH_HUMAN antibody
      • SNF2 antibody
      • SNF2 histone linker PHD RING helicase antibody
      see all

    Anti-SHPRH antibody 图像

    • Western blot - Anti-SHPRH antibody (ab80129)
      Western blot - Anti-SHPRH antibody (ab80129)This image is courtesy of an anonymous Abreview
      Anti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 193 kDa
      Observed band size : 175 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - SHPRH antibody (ab80129)
      Immunocytochemistry/ Immunofluorescence - SHPRH antibody (ab80129)
      ICC/IF image of ab80129 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml.
    • Western blot - SHPRH antibody (ab80129)
      Western blot - SHPRH antibody (ab80129)
      Anti-SHPRH antibody (ab80129) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 193 kDa
      Observed band size : 104,193 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 5 minutes      This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4 and 5) of E3 ubiquitin-protein ligase SHPRH. The predicted molecular weights of isoforms (1,2,3,4 and 5) are 193 kDa, 104kDa, 17 kDa, 190 kDa and 122 kDa respectively.

    Anti-SHPRH antibody (ab80129)参考文献

    ab80129 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab80129？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Western blot abreview for Anti-SHPRH antibody

     Good
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116 cell lines)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 30 µg
    Specification HCT116 cell lines
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C
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    Abcam user community

    Verified customer

    提交于 Jul 14 2015

    Western blot abreview for Anti-SHPRH antibody

     Excellent
    Abreviews
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Jan 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"