概述

  • 产品名称
    Anti-SHPRH抗体
    参阅全部 SHPRH 一抗
  • 描述
    兔多克隆抗体to SHPRH
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human SHPRH.

    参阅Abcam的专有抗源政策

    (Peptide available as ab89391.)

  • 阳性对照
    • This antibody gave a positive signal in SHSY-5Y whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).

靶标

  • 功能
    E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
  • 组织特异性
    Broadly expressed.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • 结构域
    The RING finger mediates E3 ubiquitin ligase activity.
  • Information by UniProt
  • 数据库链接
    • 别名
      • bA545I5.2 antibody
      • E3 ubiquitin-protein ligase SHPRH antibody
      • FLJ27258 antibody
      • FLJ37625 antibody
      • FLJ45012 antibody
      • FLJ90837 antibody
      • histone-linker antibody
      • KIAA2023 antibody
      • MGC134886 antibody
      • PHD and RING finger domain-containing helicase antibody
      • Shprh antibody
      • SHPRH_HUMAN antibody
      • SNF2 antibody
      • SNF2 histone linker PHD RING helicase antibody
      see all

    图片

    • Western blot - Anti-SHPRH antibody (ab80129)
      Western blot - Anti-SHPRH antibody (ab80129)This image is courtesy of an anonymous Abreview
      Anti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 193 kDa
      Observed band size: 175 kDa (why is the actual band size different from the predicted?)


      Exposure time: 5 minutes

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SHPRH antibody (ab80129)
      Immunocytochemistry/ Immunofluorescence - Anti-SHPRH antibody (ab80129)
      ICC/IF image of ab80129 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml.
    • Western blot - Anti-SHPRH antibody (ab80129)
      Western blot - Anti-SHPRH antibody (ab80129)
      Anti-SHPRH antibody (ab80129) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 193 kDa
      Observed band size: 104,193 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 5 minutes


      This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4 and 5) of E3 ubiquitin-protein ligase SHPRH. The predicted molecular weights of isoforms (1,2,3,4 and 5) are 193 kDa, 104kDa, 17 kDa, 190 kDa and 122 kDa respectively.

    文献

    This product has been referenced in:
    • Qu Y  et al. Axitinib blocks Wnt/ß-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A 113:9339-44 (2016). WB . Read more (PubMed: 27482107) »

    See 1 Publication for this product

    发表研究结果有使用 ab80129？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    2 reviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-SHPRH antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    HCT116 cell lines
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2015

    Western blot abreview for Anti-SHPRH antibody

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Jan 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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