- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-SHPRH抗体
参阅全部 SHPRH 一抗 -
描述兔多克隆抗体to SHPRH
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经测试应用适用于: ICC/IF, WBmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human SHPRH.
(Peptide available as ab89391.)
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阳性对照
- This antibody gave a positive signal in SHSY-5Y whole cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab80129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa). |
靶标
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功能E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
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组织特异性Broadly expressed.
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通路Protein modification; protein ubiquitination.
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序列相似性Belongs to the SNF2/RAD54 helicase family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
结构域The RING finger mediates E3 ubiquitin ligase activity.
- Information by UniProt
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数据库链接
- Entrez Gene: 257218 Human
- Omim: 608048 Human
- SwissProt: Q149N8 Human
- Unigene: 723297 Human
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别名
- bA545I5.2 antibody
- E3 ubiquitin-protein ligase SHPRH antibody
- FLJ27258 antibody
see all
Anti-SHPRH antibody 图像
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Western blot - Anti-SHPRH antibody (ab80129)This image is courtesy of an anonymous AbreviewAnti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 193 kDa
Observed band size : 175 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutesThis image is courtesy of an anonymous Abreview
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Immunocytochemistry/ Immunofluorescence - Anti-SHPRH antibody (ab80129)ICC/IF image of ab80129 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml. -
Western blot - Anti-SHPRH antibody (ab80129)Anti-SHPRH antibody (ab80129) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 193 kDa
Observed band size : 104,193 kDa (why is the actual band size different from the predicted?)
Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutesThis antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4 and 5) of E3 ubiquitin-protein ligase SHPRH. The predicted molecular weights of isoforms (1,2,3,4 and 5) are 193 kDa, 104kDa, 17 kDa, 190 kDa and 122 kDa respectively.
Anti-SHPRH antibody (ab80129)参考文献
This product has been referenced in:
- Qu Y et al. Axitinib blocks Wnt/ß-catenin signaling and directs asymmetric cell division in cancer. Proc Natl Acad Sci U S A 113:9339-44 (2016). WB . Read more (PubMed: 27482107) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"