Anti-SHP2抗体[M163] (ab76285)
Key features and details
- Mouse monoclonal [M163] to SHP2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-SHP2抗体[M163]
参阅全部 SHP2 一抗 -
描述
小鼠单克隆抗体[M163] to SHP2 -
宿主
Mouse -
特异性
ab76285 does not cross-react with SHP1. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Recombinant fragment corresponding to Human SHP2 (N terminal).
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阳性对照
- Human A431 and Jurkat cells, and adult mouse brain lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M163 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 68 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 68 kDa). |
靶标
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功能
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. -
组织特异性
Widely expressed, with highest levels in heart, brain, and skeletal muscle. -
疾病相关
Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
结构域
The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme. -
翻译后修饰
Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5781 Human
- Entrez Gene: 19247 Mouse
- Entrez Gene: 25622 Rat
- Omim: 176876 Human
- SwissProt: Q06124 Human
- SwissProt: P35235 Mouse
- SwissProt: P41499 Rat
- Unigene: 506852 Human
see all -
别名
- BPTP3 antibody
- CFC antibody
- JMML antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SHP2 knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab76285 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76285 was shown to specifically react with SHP2 when SHP2 knockout samples were used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. ab76285 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Lane 1 : Anti-SHP2 antibody [M163] (ab76285) at 1/250 dilution
Lane 2 : Anti-SHP2 antibody [M163] (ab76285) at 1/500 dilution
Lane 3 : Anti-SHP2 antibody [M163] (ab76285) at 1/1000 dilution
Lane 4 : Anti-SHP2 antibody [M163] (ab76285) at 1/2000 dilution
All lanes : adult mouse brain lysates
Predicted band size: 68 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76285 被引用在 4 文献中.
- Wang S et al. YB1 protects cardiac myocytes against H2O2-induced injury via suppression of PIAS3 mRNA and phosphorylation of STAT3. Mol Med Rep 19:4579-4588 (2019). PubMed: 30942400
- Emdal KB et al. Integrated proximal proteomics reveals IRS2 as a determinant of cell survival in ALK-driven neuroblastoma. Sci Signal 11:N/A (2018). PubMed: 30459283
- Bowen ME et al. SHP2 regulates chondrocyte terminal differentiation, growth plate architecture and skeletal cell fates. PLoS Genet 10:e1004364 (2014). WB ; Mouse . PubMed: 24875294
- Kikkawa N et al. miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC). Br J Cancer 103:877-84 (2010). WB ; Human . PubMed: 20700123