概述

  • 产品名称Anti-SHP2抗体
    参阅全部 SHP2 一抗
  • 描述
    山羊多克隆抗体to SHP2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Pig
  • 免疫原

    Synthetic peptide:

    YENVGLMQQQKSFR

    , corresponding to C terminal amino acids 580-593 of Human SHP2.

  • 阳性对照
    • Human muscle and 293 lysate.
  • 常规说明GenBank Accession Number – NP_002825.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

靶标

  • 功能Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
  • 组织特异性Widely expressed, with highest levels in heart, brain, and skeletal muscle.
  • 疾病相关Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
    Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
    Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
    Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • 结构域The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
  • 翻译后修饰Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BPTP3 antibody
    • CFC antibody
    • JMML antibody
    • METCDS antibody
    • MGC14433 antibody
    • NS1 antibody
    • OTTHUMP00000166107 antibody
    • OTTHUMP00000166108 antibody
    • Protein tyrosine phosphatase 2 antibody
    • Protein tyrosine phosphatase 2C antibody
    • Protein tyrosine phosphatase non receptor type 11 antibody
    • Protein-tyrosine phosphatase 1D antibody
    • Protein-tyrosine phosphatase 2C antibody
    • PTN11_HUMAN antibody
    • PTP-1D antibody
    • PTP-2C antibody
    • PTP1D antibody
    • PTP2C antibody
    • PTPN11 antibody
    • SAP2 antibody
    • SH-PTP2 antibody
    • SH-PTP3 antibody
    • SH2 domain containing protein tyrosine phosphatase 2 antibody
    • SHP 2 antibody
    • SHP-2 antibody
    • Shp2 antibody
    • SHPTP2 antibody
    • SHPTP3 antibody
    • Syp antibody
    • Tyrosine-protein phosphatase non-receptor type 11 antibody
    see all

Anti-SHP2 antibody 图像



  • Predicted band size : 68 kDa


    ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab9214 at 3.8 µg/ml staining SHP2 in Human cerebellum by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-SHP2 antibody (ab9214)参考文献

This product has been referenced in:
  • Liu HP  et al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB ; Human . Read more (PubMed: 21070852) »
  • Takagi K  et al. Modulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways. J Immunol 174:1801-10 (2005). WB ; Mouse . Read more (PubMed: 15699106) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab9214.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"