Anti-SHP1 (phospho Y536)抗体(ab51171)

概述

  • 产品名称Anti-SHP1 (phospho Y536)抗体
    参阅全部 SHP1 一抗
  • 描述
    兔多克隆抗体to SHP1 (phospho Y536)
  • 特异性ab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

  • 阳性对照
    • Human breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).

性能

应用

Our Abpromise guarantee covers the use of ab51171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Predicted molecular weight: 68 kDa.

靶标

  • 功能Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • 组织特异性Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻译后修饰Phosphorylated on serine and tyrosine residues.
  • 细胞定位Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Anti-SHP1 (phospho Y536) antibody 图像

  • ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.


  • Predicted band size : 68 kDa


    Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)

Anti-SHP1 (phospho Y536) antibody (ab51171)参考文献

This product has been referenced in:
  • Abbonante V  et al. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem 288:16738-46 (2013). Human . Read more (PubMed: 23530036) »
  • Ketroussi F  et al. Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway. PLoS One 6:e22776 (2011). WB ; Human . Read more (PubMed: 21887223) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Jurkat)
Specification Jurkat
Preparation Cell harvesting/tissue preparation method: human Jurkat cells treated with pervanadate (1 mM) for 30 min
Sample buffer: complete RPMI media
Fixation Paraformaldehyde
Permeabilization Yes - methanol
Gating Strategy Jurkat
Username

Abcam user community

Verified customer

提交于 Feb 18 2013

Thank you foryour reply.

The buffers for these product do not contain stabilizing proteins.



Ab51171: Preservative: 0.02% Sodium Azide

Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium ...

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Thank you for your reply.

Unfortunately none of these products has yet been tested in flow cytometry. While we do try and test as many products as we can in a large number of applications, it does seem as this group has not yet been though...

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Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numbera...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"