The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. Contains 2 SH2 domains. Contains 1 tyrosine-protein phosphatase domain.
Phosphorylated on serine and tyrosine residues.
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
ICC/IF image of ab41437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41437 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - SHP1 (phospho Y536) antibody (ab41437)
Lane 1 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lane 2 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
Lane 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lane 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
with phospho-Tyr536-SHP1 peptide
Predicted band size : 68 kDa Observed band size : 68 kDa Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Zhou X et al. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. Am J Physiol Renal Physiol307:F516-24 (2014).
Read more (PubMed: 24990897) »
Kim KA et al. Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica. Parasite Immunol32:176-83 (2010).
Read more (PubMed: 20398180) »