Anti-SHP1 (phospho Y536)抗体(ab41437)
Key features and details
- Rabbit polyclonal to SHP1 (phospho Y536)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SHP1 (phospho Y536)抗体
参阅全部 SHP1 一抗 -
描述
兔多克隆抗体to SHP1 (phospho Y536) -
宿主
Rabbit -
特异性
Phospho-Tyr536-SHP1 specific. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate (not observed in control cells). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human SHP1 (phospho Y536).
Database link: P29350 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was cross-adsorbed to a non specific phospho-tyrosine peptide then affinity purified using phospho-Tyr536-SHP1 peptide (immunogen). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41437于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
靶标
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功能
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
组织特异性
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Phosphorylated on serine and tyrosine residues. -
细胞定位
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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数据库链接
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Entrez Gene: 116689 Rat
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- SwissProt: P81718 Rat
- Unigene: 63489 Human
see all -
别名
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
图片
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Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-5 : Anti-SHP1 (phospho Y536) antibody (ab41437) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
with phospho-Tyr536-SHP1 peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab41437 被引用在 3 文献中.
- Ormonde JVS et al. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK. J Immunol 201:3431-3442 (2018). PubMed: 30373850
- Zhou X et al. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. Am J Physiol Renal Physiol 307:F516-24 (2014). PubMed: 24990897
- Kim KA et al. Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica. Parasite Immunol 32:176-83 (2010). WB ; Human . PubMed: 20398180