Anti-SHP1抗体[M160] (ab76202)

概述

  • 产品名称Anti-SHP1抗体[M160]
    参阅全部 SHP1 一抗
  • 描述
    小鼠单克隆抗体[M160] to SHP1
  • 特异性ab76202 does not cross-react with SHP2.
  • 经测试应用适用于: WB, IP, ELISA, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant protein containing amino acids in the C terminal region of human SHP1.

  • 阳性对照
    • Jurkat cells treated with pervanadate (1 mM) for 30 min.

性能

应用

Our Abpromise guarantee covers the use of ab76202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP 1/100.
ELISA 1/2000.
ICC 1/100.

靶标

  • 功能Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • 组织特异性Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • 翻译后修饰Phosphorylated on serine and tyrosine residues.
  • 细胞定位Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Anti-SHP1 antibody [M160] 图像

  • All lanes : Anti-SHP1 antibody [M160] (ab76202) at 1/500 dilution

    Lane 1 : Jurkat cells treated with pervanadate (1 mM) for 30 min
    Lane 2 : Jurkat cells treated with pervanadate (1 mM) for 30 min and treated with lambda phosphatase


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

Anti-SHP1 antibody [M160] (ab76202)参考文献

ab76202 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Differentiated 3T3-L1 adipocytes)
Gel Running Conditions Reduced Denaturing (8.5%)
Loading amount 30 µg
Treatment Transfected with scramble or Shp1 shRNA
Specification Differentiated 3T3-L1 adipocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Aug 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"