Anti-SHMT2抗体(ab88664)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to SHMT2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human SHMT2 protein (NP_005403.2).

  • 阳性对照
    • Human spleen tissue lysate. Hela cell lysate. SHMT2 transfected 293T cell lysate. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • 通路
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 序列相似性
    Belongs to the SHMT family.
  • 细胞定位
    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis secretory sperm binding protein Li 51e antibody
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • HEL S 51e antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

图片

  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SHMT2 antibody (ab88664) at 1 µg/ml

    Lane 1 : SHMT2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab88664, at 10 µg/ml, staining SHMT2 in Hela cells by Immunofluorescence.

文献

ab88664 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
20 µg
Specification
Jurkat
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

提交于 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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