概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to SHMT1
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 374-483 of Human SHMT1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab55736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Interconversion of serine and glycine.
  • 通路
    One-carbon metabolism; tetrahydrofolate interconversion.
  • 序列相似性
    Belongs to the SHMT family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSHMT antibody
    • Cytoplasmic serine hydroxymethyltransferase antibody
    • cytosolic antibody
    • GLYC_HUMAN antibody
    • Glycine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase 1 (soluble) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase, cytosolic antibody
    • Serine methylase antibody
    • SHMT antibody
    • Shmt1 antibody
    see all

Anti-SHMT1 antibody 图像

  • Overlay histogram showing HeLa cells stained with ab55736 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55736, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • SHMT1 antibody (ab55736) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.


  • Predicted band size : 53 kDa
    SHMT1 antibody (ab55736) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-SHMT1 antibody (ab55736)参考文献

This product has been referenced in:
  • Krall AS  et al. Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. Nat Commun 7:11457 (2016). WB . Read more (PubMed: 27126896) »
  • Kim SK  et al. Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes. PLoS One 9:e101004 (2014). WB ; Human . Read more (PubMed: 24979213) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

提交于 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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