概述

  • 产品名称Anti-SHIP抗体[SHIP-01]
    参阅全部 SHIP 一抗
  • 描述
    小鼠单克隆抗体[SHIP-01] to SHIP
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a sequence within the N-terminal domain of Human SHIP.

  • 阳性对照
    • RAMOS human cell line. Whole cell lysate of THP-1 human acute monocytic leukemia cell line.

性能

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应用

Our Abpromise guarantee covers the use of ab65807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects bands of approximately 110, 125, 135 & 145 kDa (predicted molecular weight: 133 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
    • 组织特异性Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
    • 序列相似性Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
      Contains 1 SH2 domain.
    • 结构域The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation.
      The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.
    • 翻译后修饰Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
    • 细胞定位Cytoplasm. Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Inositol polyphosphate 5 phosphatase of 145kDa antibody
      • 4 antibody
      • 5-trisphosphate 5-phosphatase 1 antibody
      • hp51CN antibody
      • Inositol polyphosphate 5 phosphatase 145kDa antibody
      • Inositol polyphosphate 5 phosphatase antibody
      • Inositol polyphosphate-5-phosphatase of 145 kDa antibody
      • INPP5D antibody
      • MGC104855 antibody
      • MGC142140 antibody
      • MGC142142 antibody
      • p150Ship antibody
      • Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
      • Phosphatidylinositol-3 antibody
      • SH2 containing inositol phosphatase isoform b antibody
      • SH2 domain containing inositol 5' phosphatase 1 antibody
      • SH2 domain containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol-5''-phosphatase 1 antibody
      • SHIP-1 antibody
      • SHIP1 antibody
      • SHIP1_HUMAN antibody
      • Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
      • SIP-145 antibody
      • SIP145 antibody
      see all

    Anti-SHIP antibody [SHIP-01] 图像

    • Lane 1 : Isotype mouse IgG1 control
      Lanes 2 - 3 : Anti-SHIP antibody [SHIP-01] (ab65807)

      Lane 1 : whole cell lysate of THP-1 human acute monocytic leukemia cell line.
      Lane 2 : whole cell lysate of THP-1 human acute monocytic leukemia cell line.
      Lane 3 : whole cell lysate of THP-1 human acute monocytic leukemia cell line.


      Predicted band size : 133 kDa
      Observed band size : 110,125,135,145 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Anti-SHIP antibody [SHIP-01] (ab65807)参考文献

    This product has been referenced in:
    • Lo TC  et al. Inactivation of SHIP1 in T-cell acute lymphoblastic leukemia due to mutation and extensive alternative splicing. Leuk Res 33:1562-6 (2009). Read more (PubMed: 19473701) »

    See 1 Publication for this product

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