The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
See attached protocol.
Shigella sonnei Phase II belongs to the Enterobacteriaceae family. It is a nonmotile gram negative bacillus. The organism invades the epithelial cell of the large intestine which causes shigellosis with mucous and bloody diarrhea, fever and abdominal pain.
Shingella is classified into the 4 following species, by serological properties of somatic antigens (O) antigens and biochemical properties according to the recommendation of the International Enterbacteriaceae Grouping Subcommitte (1984); S.dysentarias (subgroup A), S.Flexneri (subgroup B), S.boydii (subgroup C) and S.sonnei (subgroup D). There is one serotype for S.sonnei classified into 2 antigens, phase I (smooth: S type) and Phase II (rough: R type) according to the S-R mutation of the O antigen.