Anti-Shigella抗体(HRP) (ab19952)

概述

  • 产品名称Anti-Shigella抗体(HRP)
    参阅全部 Shigella 一抗
  • 描述
    兔多克隆抗体to Shigella (HRP)
  • 偶联物HRP
  • 特异性Rabbit polyclonal to Shigella (HRP)(ab19952) is polyvalent for Shigella antigens. It reacts with Shigella species including sonnei. Antiserum is not absorbed.
  • 经测试应用适用于: ICC/IFmore details
  • 种属反应性
    Reacts with related Enterobacteriaceae. Not yet tested in other species.
  • 免疫原

    Mixture of S. boydii (ATCC #8700), S. flexneri (ATCC #29903) and S. dysenteriae (ATCC #13313)

  • 常规说明

    Rabbit polyclonal to Shigella (HRP) consists of the purified IgG fraction covalently coupled with highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Estimated molar HRP: IgG substitution is 2-3.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Free horseradish peroxidase enzyme is not present.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
  • 应用说明IF: Use at an assay dependent dilution.
    Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% Acetone for 5 minutes at room temperature. Air dry. Stain.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals. Shigella species are classified by four serogroups: * Serogroup A: S. dysenteriae (12 serotypes) * Serogroup B: S. flexneri (6 serotypes) * Serogroup C: S. boydii (23 serotypes) * Serogroup D: S. sonnei (1 serotype) Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.
    • 别名
      • S. boydii antibody
      • S. dysenteriae antibody
      • S. flexneri antibody
      • S. sonnei antibody
      • Shigella Boydii antibody
      • Shigella Dysenteriae antibody
      • Shigella Flexneri antibody
      • Shigella Sonnei antibody
      see all

    Anti-Shigella antibody (HRP) (ab19952)参考文献

    ab19952 has not yet been referenced specifically in any publications.

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    I am sorry to hear that you have been experiencing issues when using this product in ELISA. As stated on the datasheet of this product; Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-sh...

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