羊Anti-Mouse IgG H&L (HRP) (ab102458)

概述

  • 产品名称羊Anti-Mouse IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 靶标种属Mouse
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • 经测试应用适用于: ICC, ELISA, IHC-P, WBmore details
  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000. (Primary)
IHC-P 1/200 - 1/5000.
WB 1/10000 - 1/50000. with a chemoluminescence detection method. If using a colorimetric method, dilute 1/5000 - 1/30000.

Sheep Anti-Mouse IgG H&L (HRP) (ab102458)参考文献

ab102458 has not yet been referenced specifically in any publications.

Product Wall

Hi, glad to be of help, and Eve is REALLY glad you liked the brownies!

I have attached our dot blot protocol. However, on second thought, it may not be applicable to a blot of just the secondary, because I am not sure how well the HRP will handle the drying step that is at at the start of the attached protocol, after appl...

Read More

Thank you for bringing this to our attention. It could very well be that the HRP is the issue, or the antibody:antibody affinity. I am assuming you are using the same primary with both secondaries (the more expensive one from another company and ours)....

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"