antigen sequence, corresponding to internal sequence amino acids 6-139 of Human SHD.
- Human heart muscle tissue; RT4 and U 251 MG cell lysate; Human Liver and Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May function as an adapter protein.
Contains 1 SH2 domain.
Tyrosine phosphorylated by ABL.
Information by UniProt
- SH2 domain-containing adapter protein D antibody
- SHD antibody
- SHD_HUMAN antibody
- Src homology 2 domain containing transforming protein D antibody
Immunocytochemistry/ Immunofluorescence - Anti-SHD antibody (ab121544)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121544 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHD antibody (ab121544)
ab121544 at 1/200 dilution staining SHD in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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