RabMAb

Anti-SHC (phospho Y427)抗体[EPR778(2)Y] (ab68166)

概述

  • 产品名称Anti-SHC (phospho Y427)抗体[EPR778(2)Y]
    参阅全部 SHC 一抗
  • 描述
    兔单克隆抗体[EPR778(2)Y] to SHC (phospho Y427)
  • 经测试应用适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt,IHC-Fr,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 427 of Human SHC.

  • 阳性对照
    • A431 cells treated with EGF
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab68166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 46, 52, 66 kDa (predicted molecular weight: 63 kDa).
  • 应用说明Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
  • 靶标

    • 功能Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
    • 组织特异性Widely expressed. Expressed in neural stem cells but absent in mature neurons.
    • 序列相似性Contains 1 PID domain.
      Contains 1 SH2 domain.
    • 结构域In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation.
    • 翻译后修饰Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity). Tyrosine phosphorylated in response to FLT3 signaling (By similarity). Tyrosine phosphorylated by activated PTK2B/PYK2 (By similarity). Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1; tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.
    • 细胞定位Cytoplasm; Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization and Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ26504 antibody
      • p66 antibody
      • p66SHC antibody
      • SH2 domain protein C1 antibody
      • SHC (Src homology 2 domain containing) transforming protein 1 antibody
      • SHC 1 antibody
      • SHC A antibody
      • Shc antibody
      • SHC transforming protein 1 antibody
      • SHC transforming protein antibody
      • SHC-transforming protein 1 antibody
      • SHC-transforming protein 3 antibody
      • SHC-transforming protein A antibody
      • SHC1 antibody
      • SHC1_HUMAN antibody
      • SHCA antibody
      • Src homology 2 domain-containing-transforming protein C1 antibody
      see all

    Anti-SHC (phospho Y427) antibody [EPR778(2)Y] 图像

    • All lanes : Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166) at 1/500 dilution

      Lane 1 : A431 lysate
      Lane 2 : A431 lysate - EGF treated

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 46 kDa (possible isoform),52 kDa (possible isoform).

    Anti-SHC (phospho Y427) antibody [EPR778(2)Y] (ab68166)参考文献

    ab68166 has not yet been referenced specifically in any publications.

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