Full length human SHARPIN protein (AAH25244.1, 1 a.a. ~ 326 a.a)
- SHARPIN transfected 293T cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).
May have a role in normal immune development and control of inflammation.
Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Contains 1 RanBP2-type zinc finger.
Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Information by UniProt
- DKFZp434N1923 antibody
- hSIPL1 antibody
- Shank associated RH domain interacting protein antibody
Western blot - Anti-SHARPIN antibody (ab69507)
All lanes :
Anti-SHARPIN antibody (ab69507) at 1/500 dilutionLane 1 :
SHARPIN transfected 293T cell lysateLane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.SecondaryAll lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size:
34 kDaObserved band size:
36 kDa (why is the actual band size different from the predicted?
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