产品名称 Anti-SHARPIN抗体 参阅全部 SHARPIN 一抗
WB, IP more details
Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human SHARPIN (NP_112236.3).
HeLa, Jurkat and 293T whole cell lysates
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 40 kDa.
Use at 2-10 µg/mg of lysate.
功能 May have a role in normal immune development and control of inflammation.
组织特异性 Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
序列相似性 Contains 1 RanBP2-type zinc finger.
细胞定位 Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Information by UniProt
Shank associated RH domain interacting protein antibody
Anti-SHARPIN antibody 图像
Western blot - Anti-SHARPIN antibody (ab125188)
All lanes : Anti-SHARPIN antibody (ab125188) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg developed using the ECL technique Predicted band size : 40 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-SHARPIN antibody (ab125188)
ab125188, at 6 µg/mg lysate, detecting SHARPIN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation. For the subsequent Western blot, ab125188 was used at 1 µg/ml. The blot was developed using the ECL technique, with an exposure time of 30 seconds.
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