概述

  • 产品名称Anti-SHARP2抗体
    参阅全部 SHARP2 一抗
  • 描述
    兔多克隆抗体to SHARP2
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and the C terminus (residue 412) of human SHARP2 (NP_003661.1).

  • 阳性对照
    • Whole cell lysates from HeLa and 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab70723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
IP Use at 1-4 µg/mg of lysate.

靶标

  • 相关性SHARP2 (enhancer–of-split and hairy-related protein, differentially expressed in chondrocytes protein 1, DEC1) is a class B basic helix-loop-helix protein. Function: May function as a transcriptional factor for neuronal differentiation. PTM: Ubiquitinated; which may lead to proteasomal degradation
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • BHLHB2 antibody
    • bHLHe40 antibody
    • Class B Basic Helix Loop Helix Protein 2 antibody
    • Class E basic helix loop helix protein 40 antibody
    • Clast5 antibody
    • DEC1 antibody
    • Differentially expressed in chondrocytes protein 1 antibody
    • E47 interaction protein 1 antibody
    • EIP1 antibody
    • Enhancer of split and hairy related protein 2 antibody
    • SHARP 2 antibody
    • Stimulated by retinoic acid gene 13 protein antibody
    • STRA13 antibody
    see all

Anti-SHARP2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2 with ab70723 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-SHARP2 antibody (ab70723) at 0.1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human SHARP2 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds

Anti-SHARP2 antibody (ab70723)参考文献

ab70723 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70723.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"