重组Anti-SHANK3抗体[S69] (ab93607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [S69] to SHANK3
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-SHANK3抗体[S69]
参阅全部 SHANK3 一抗 -
描述
小鼠单克隆抗体[S69] to SHANK3 -
宿主
Mouse -
特异性
No cross-reactivity with SHANK1 or SHANK2. -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Horse, Cow -
免疫原
Synthetic peptide corresponding to Rat SHANK3 aa 800-900 (internal sequence).
Database link: Q9JLU4 -
常规说明
The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
S69 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93607于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 186 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 186 kDa). |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. -
组织特异性
Expressed in the cerebral cortex and the cerebellum. -
疾病相关
Note=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
序列相似性
Contains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
细胞定位
Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells. - Information by UniProt
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数据库链接
- Entrez Gene: 85358 Human
- Entrez Gene: 58234 Mouse
- Entrez Gene: 59312 Rat
- Omim: 606230 Human
- SwissProt: Q9BYB0 Human
- SwissProt: Q4ACU6 Mouse
- SwissProt: Q9JLU4 Rat
- Unigene: 149035 Human
see all -
别名
- AI841104 antibody
- DEL22q13.3 antibody
- KIAA1650 antibody
see all
图片
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Anti-SHANK3 antibody [S69] (ab93607) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG-HRP
Predicted band size: 186 kDa -
Immunohistochemical analysis of mouse backskin tissue sections labling SHANK3 with ab93607 at 1/100 dilution. FITC Goat anti-mouse at 1/50 was used as the secondary antibody.
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Overlay histogram showing SH-SY5Y cells stained with ab93607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab93607 被引用在 4 文献中.
- Chen B et al. SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2. Cell Death Dis 13:403 (2022). PubMed: 35468874
- Man W et al. SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction. Front Physiol 11:1082 (2020). PubMed: 32982797
- Gassowska-Dobrowolska M et al. Prenatal Exposure to Valproic Acid Affects Microglia and Synaptic Ultrastructure in a Brain-Region-Specific Manner in Young-Adult Male Rats: Relevance to Autism Spectrum Disorders. Int J Mol Sci 21:N/A (2020). PubMed: 32443651
- Zhang Y et al. Effect of intraperitoneal or intracerebroventricular injection of streptozotocin on learning and memory in mice. Exp Ther Med 16:2375-2380 (2018). PubMed: 30186480