Anti-SHANK2抗体[N23B/49] (ab94575)

概述

  • 产品名称
    Anti-SHANK2抗体[N23B/49]
    参阅全部 SHANK2 一抗
  • 描述
    小鼠单克隆抗体[N23B/49] to SHANK2
  • 特异性
    Recognizes SHANK1, SHANK2, SHANK3.
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 84-309 (SH3/PDZ domains) of Rat SHANK2 (Q9QX74).

  • 阳性对照
    • Rat brain lysate
  • 常规说明

    The clone number has been updated from S23B-49 to N23B/49, both clone numbers name the same antibody clone.

     

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    N23B/49
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • 应用说明
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 159 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
    • 组织特异性
      Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
    • 序列相似性
      Belongs to the SHANK family.
      Contains 1 PDZ (DHR) domain.
      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH3 domain.
    • 发展阶段
      Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
    • 结构域
      The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
    • 细胞定位
      Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AUTS17 antibody
      • Cortactin binding protein 1 antibody
      • Cortactin SH3 domain-binding protein antibody
      • Cortactin-binding protein 1 antibody
      • CortBP1 antibody
      • CTTNBP1 antibody
      • GKAP/SAPAP interacting protein antibody
      • GKAP/SAPAP-interacting protein antibody
      • KIAA1022 antibody
      • Proline rich synapse associated protein 1 antibody
      • Proline-rich synapse-associated protein 1 antibody
      • ProSAP1 antibody
      • SH3 and multiple ankyrin repeat domains 2 antibody
      • SH3 and multiple ankyrin repeat domains protein 2 antibody
      • SHAN2_RAT antibody
      • SHANK antibody
      • Shank2 antibody
      • SPANK-3 antibody
      see all

    图片



    • Predicted band size : 159 kDa
      Western blot detection of SHANK2 in Rat braim membrane lysates using ab94575 at 1:1000 dilution.

    文献

    ab94575 has not yet been referenced specifically in any publications.

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