The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: > 1/200.
WB: > 1/500. Detects a band of approximately 63-65 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Shadow protein belongs to the cytochrome P450 family. It is required for CNS development of midline glial cells and is involved in the metabolism of insect hormones. It is responsible for ecdysteroid C2-hydroxylase activity and may be involved in the breakdown of synthetic insecticides.