概述

  • 产品名称Anti-SH3PX1抗体[2F1]
    参阅全部 SH3PX1 一抗
  • 描述
    小鼠单克隆抗体[2F1] to SH3PX1
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human, Monkey
  • 免疫原

    Recombinant full length Human SH3PX1 produced in HEK293T cells (NP_057308).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY SH3PX1 cDNA; HepG2, HeLa, HT29, A549, Jurkat, COS7 and MCF7 cell extracts; COS7 cells transiently transfected by pCMV6-ENTRY SH3PX1; HEK293T cells transfected with pCMV6-ENTRY SH3PX1 overexpress plasmid; Jurkat cells.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab118996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 67 kDa.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.
  • 序列相似性Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 SH3 domain.
  • 结构域The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol-4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates.
  • 翻译后修饰Ubiquitinated by ITCH.
    Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.
  • 细胞定位Cytoplasmic vesicle membrane. Cell membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network. Cell projection > ruffle. Cytoplasm. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MST 155 antibody
    • MST155 antibody
    • MSTP 155 antibody
    • MSTP155 antibody
    • OTTHUMP00000040083 antibody
    • Protein SDP 1 antibody
    • Protein SDP1 antibody
    • SDP 1 antibody
    • SDP1 antibody
    • SH3 and PX domain containing protein 3A antibody
    • SH3 and PX domain containing protein SH3PX1 antibody
    • SH3 and PX domain-containing protein 1 antibody
    • SH3 and PX domain-containing protein 3A antibody
    • SH3PX1 antibody
    • SH3PXD3A antibody
    • SNX 9 antibody
    • SNX9 antibody
    • SNX9_HUMAN antibody
    • Sorting nexin 9 antibody
    • Sorting nexin-9 antibody
    • WASP interactor protein antibody
    • Wiskott Aldrich syndrome protein (WASP) interactor protein antibody
    • Wiskott Aldrich syndrome protein interactor protein antibody
    • WISP antibody
    see all

Anti-SH3PX1 antibody [2F1] 图像



  • Predicted band size : 67 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SH3PX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HCT116 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab118996 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab118996 was shown to specifically react with SH3PX1 when SH3PX1 knockout samples were used. Wild-type and SH3PX1 knockout samples were subjected to SDS-PAGE. ab118996 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-SH3PX1 antibody [2F1] (ab118996) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SH3PX1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 67 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202822)
  • All lanes : Anti-SH3PX1 antibody [2F1] (ab118996) at 1/1000 dilution

    Lane 1 : HepG2 cell line extract
    Lane 2 : HeLa cell line extract
    Lane 3 : HT29 cell line extract
    Lane 4 : A549 cell line extract
    Lane 5 : COS7 cell line extract
    Lane 6 : Jurkat cell line extract
    Lane 7 : MDCK cell line extract
    Lane 8 : PC12 cell line extract
    Lane 9 : MCF7 cell line extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 67 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202822)
  • ab118996, at 1/100 dilution staining SH3PX1 in COS7 cells transiently transfected with pCMV6-ENTRY SH3PX1 by Immunofluorescence.
  • ab118996, at 1/100, staining SH3PX1 in HEK293T cells transfected with either SH3PX1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry.
  • ab118996, at 1/100, staining SH3PX1, in Jurkat cells by Flow cytometry (Red), compared to a nonspecific negative control antibody (Blue).

Anti-SH3PX1 antibody [2F1] (ab118996)参考文献

This product has been referenced in:
  • Lettau M  et al. Differential protein-protein interactions of full length human FasL and FasL fragments generated by proteolysis. Exp Cell Res 320:290-301 (2014). Human . Read more (PubMed: 24291222) »

See 1 Publication for this product

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