概述

  • 产品名称Anti-SH3BP5抗体
    参阅全部 SH3BP5 一抗
  • 描述
    小鼠单克隆抗体to SH3BP5
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 4-113 of Human SH3BP5

  • 阳性对照
    • ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab56603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
  • 组织特异性Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
  • 序列相似性Belongs to the SH3BP5 family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3BP5_HUMAN antibody
    • OTTHUMP00000208289 antibody
    • OTTHUMP00000208291 antibody
    • OTTHUMP00000208294 antibody
    • SAB antibody
    • SH3 binding protein antibody
    • SH3 domain binding protein 5 (BTK associated) antibody
    • SH3 domain binding protein 5 antibody
    • SH3 domain binding protein that preferentially associates with BTK antibody
    • SH3 domain-binding protein 5 antibody
    • SH3 domain-binding protein that preferentially associates with BTK antibody
    • SH3 domainbinding protein 5 antibody
    • SH3BP 5 antibody
    • SH3BP-5 antibody
    • Sh3bp5 antibody
    see all

Anti-SH3BP5 antibody 图像

  • ICC/IF image of ab56603 stained HeLa cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56603, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • SH3BP5 antibody (ab56603) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon adenocarcinoma.


  • Predicted band size : 47 kDa
    SH3BP5 antibody (ab56603) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab56603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56603, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-SH3BP5 antibody (ab56603)参考文献

ab56603 has not yet been referenced specifically in any publications.

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