概述

  • 产品名称Anti-SH3BGRL抗体
    参阅全部 SH3BGRL 一抗
  • 描述
    兔多克隆抗体to SH3BGRL
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region within amino acids 66-115 of Human SH3BGRL (LPPQIFNESQ YRGDYDAFFE ARENNAVYAF LGLTAPPGSK EAEVQAKQQA)

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

Anti-SH3BGRL antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SH3BGRL with ab80865 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SH3BGRL. Right - Merge.
  • Anti-SH3BGRL antibody (ab80865) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000.
    Developed using the ECL technique

    Predicted band size : 13 kDa

Anti-SH3BGRL antibody (ab80865)参考文献

ab80865 has not yet been referenced specifically in any publications.

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