概述
- 产品名称Anti-SH3 containing Grb 2 like 1 protein抗体
参阅全部 SH3 containing Grb 2 like 1 protein 一抗 - 描述兔多克隆抗体to SH3 containing Grb 2 like 1 protein
- 经测试应用适用于: WB, IPmore details
- 种属反应性与反应: Human
预测可用于: Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
- 免疫原
Synthetic peptide, corresponding to a region between residues 225 and 275 of human SH3 containing Grb 2 like 1 protein (NP_003016.1)
- 阳性对照
- HeLa whole cell lysate.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab86605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
靶标
- 功能Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
- 组织特异性Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
- 疾病相关Note=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
- 序列相似性Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain. - 结构域An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
- 细胞定位Cytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
- Information by UniProt
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数据库链接
- Entrez Gene: 485039 Dog
- Entrez Gene: 6455 Human
- Omim: 601768 Human
- SwissProt: Q99961 Human
- Unigene: 97616 Human
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别名
- CNSA1 antibody
- EEN antibody
- EEN fusion partner of MLL antibody
see all
Anti-SH3 containing Grb 2 like 1 protein antibody 图像
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Western blot - SH3 containing Grb 2 like 1 protein antibody (ab86605)All lanes : Anti-SH3 containing Grb 2 like 1 protein antibody (ab86605) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 41 kDa
Exposure time : 30 seconds -
Immunoprecipitation - SH3 containing Grb 2 like 1 protein antibody (ab86605)Detection of SH3 containing Grb 2 like 1 protein by Western Blot of Immunprecipitate.
ab86605 at 1µg/ml staining SH3 containing Grb 2 like 1 protein in HeLa whole cell lysate immunoprecipitated using ab86605 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-SH3 containing Grb 2 like 1 protein antibody (ab86605)参考文献
ab86605 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"