概述

  • 产品名称Anti-SH2B1抗体
    参阅全部 SH2B1 一抗
  • 描述
    小鼠单克隆抗体to SH2B1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: GKAKHLRLSL NEEGQCRVQH LWFQSIFDML EHFRVHPIPL ESGGSSDVVL VSYVPSSQRQ QGREQAGSHA GVCEGDGCHP DASCTLMPFG ASDCVTDHLP , corresponding to amino acids 327-426 of Human SH2B

性能

应用

Our Abpromise guarantee covers the use of ab57161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis.
    • 组织特异性Widely expressed with highest levels in skeletal muscle and ovary.
    • 序列相似性Belongs to the SH2B adapter family.
      Contains 1 PH domain.
      Contains 1 SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET.
    • 细胞定位Cytoplasm. Membrane. Nucleus. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp547G1110 antibody
      • FLJ30542 antibody
      • KIAA1299 antibody
      • PH and SH2 domain-containing signaling mediator antibody
      • Pro-rich antibody
      • Pro-rich, PH and SH2 domain-containing signaling mediator antibody
      • PSM antibody
      • SH2 B antibody
      • SH2 domain-containing protein 1B antibody
      • SH2 domain-containing putative adapter SH2-B antibody
      • SH2-B signaling protein antibody
      • SH2B antibody
      • SH2B adapter protein 1 antibody
      • SH2B adaptor protein 1 antibody
      • Sh2b1 antibody
      • SH2B1 protein antibody
      • SH2B1_HUMAN antibody
      see all

    Anti-SH2B1 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57161 SH2B antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-SH2B1 antibody (ab57161)参考文献

    ab57161 has not yet been referenced specifically in any publications.

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