Small glutamine rich protein with tetratricopeptide repeats 1 antibody
Small glutamine rich tetratricopeptide repeat containing protein alpha antibody
Small glutamine rich tetratricopeptide repeat TPR containing alpha antibody
Small glutamine rich tetratricopeptide repeat-containing protein antibody
Small glutamine-rich tetratricopeptide repeat-containing protein alpha antibody
Vpu binding protein antibody
Vpu-binding protein antibody
Anti-SGTA antibody 图像
Flow Cytometry - Anti-SGTA antibody (ab55689)
Overlay histogram showing HeLa cells stained with ab55689 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55689, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - SGTA antibody (ab55689)
Predicted band size : 34 kDa SGTA antibody (ab55689) at 1ug/lane + HeLa cell lysate at 25ug/lane.