概述

  • 产品名称Anti-SGT1抗体
    参阅全部 SGT1 一抗
  • 描述
    兔多克隆抗体to SGT1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 450 and 500 of human SGT1 (NP_009196.1)

  • 阳性对照
    • HeLa and 293T whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab99293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

Anti-SGT1 antibody 图像

  • All lanes : Anti-SGT1 antibody (ab99293) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 73 kDa


    Exposure time : 3 seconds
  • Lane 1 : Detection of Human SGT1 by Immunoprecipitation from Hela ( 1 mg for IP, 20% of IP loaded), using 3µg/mg lysate. Lane 2 : Control IgG

Anti-SGT1 antibody (ab99293)参考文献

ab99293 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NIH-3T3
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"