概述

  • 产品名称Anti-SGT1抗体
    参阅全部 SGT1 一抗
  • 描述
    兔多克隆抗体to SGT1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (PAHMFGVTKF GDNIEDEWFI VYVIKQITKE FPELVARIED NDGEFLLIEA) of human SGT1 (NP_009196).

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

靶标

Anti-SGT1 antibody 图像

  • Anti-SGT1 antibody (ab82956) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

Anti-SGT1 antibody (ab82956)参考文献

ab82956 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NIH-3T3
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Dec 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"