概述

  • 产品名称
  • 描述
    兔多克隆抗体to SGPL1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to C-terminal residues of mouse SGPL1.

性能

应用

Our Abpromise guarantee covers the use of ab56183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.
  • 组织特异性
    Found in liver and kidney.
  • 通路
    Lipid metabolism; sphingolipid metabolism.
  • 序列相似性
    Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ13811 antibody
    • hSPL antibody
    • KIAA1252 antibody
    • S1P antibody
    • S1PL antibody
    • SGPL 1 antibody
    • SGPL1 antibody
    • SGPL1_HUMAN antibody
    • SP lyase antibody
    • SP-lyase 1 antibody
    • Sphingosine 1 phosphate aldolase antibody
    • Sphingosine 1 phosphate lyase 1 antibody
    • Sphingosine-1-phosphate aldolase antibody
    • Sphingosine-1-phosphate lyase 1 antibody
    • SPL 1 antibody
    • SPL antibody
    see all

实验方案

文献

This product has been referenced in:
  • Hagen N  et al. Sphingosine-1-phosphate links glycosphingolipid metabolism to neurodegeneration via a calpain-mediated mechanism. Cell Death Differ 18:1356-65 (2011). IHC (PFA fixed) ; Mouse . Read more (PubMed: 21331079) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Username

Anabel Delagarza

Verified customer

提交于 Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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