概述

  • 产品名称
  • 描述
    兔多克隆抗体to SGPL1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SGPL1.

  • 阳性对照
    • This antibody gave a positive control in both Human Kidney and Liver tissue lysates as well as HepG2 and HEK293 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab105183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

靶标

  • 功能
    Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.
  • 组织特异性
    Found in liver and kidney.
  • 通路
    Lipid metabolism; sphingolipid metabolism.
  • 序列相似性
    Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ13811 antibody
    • hSPL antibody
    • KIAA1252 antibody
    • S1P antibody
    • S1PL antibody
    • SGPL 1 antibody
    • SGPL1 antibody
    • SGPL1_HUMAN antibody
    • SP lyase antibody
    • SP-lyase 1 antibody
    • Sphingosine 1 phosphate aldolase antibody
    • Sphingosine 1 phosphate lyase 1 antibody
    • Sphingosine-1-phosphate aldolase antibody
    • Sphingosine-1-phosphate lyase 1 antibody
    • SPL 1 antibody
    • SPL antibody
    see all

Anti-SGPL1 antibody 图像

  • All lanes : Anti-SGPL1 antibody (ab105183) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

实验方案

Anti-SGPL1 antibody (ab105183)参考文献

This product has been referenced in:
  • Didonna A  et al. A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Hum Mol Genet 24:7151-8 (2015). Read more (PubMed: 26433934) »

See 1 Publication for this product

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