概述

  • 产品名称
    Anti-SGOL2抗体
  • 描述
    兔多克隆抗体to SGOL2
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: ESHSHSDQSS KTSLMSEMRN AQSIGRRWEK PSPSNVTERK KRGSSWESNN LSADTPCATV LDKQHISSPE LNCNNEINGH TNETNTEMQR N, corresponding to amino acids 235-325 of Human SGOL2.

  • 阳性对照
    • Human testis tissue; A431 cells.

性能

应用

Our Abpromise guarantee covers the use of ab122258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 145 kDa.

靶标

  • 功能
    Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.
  • 序列相似性
    Belongs to the shugoshin family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. During meiosis I, accumulates at centromeres during diplotene, and co-localizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost. During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity). In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase. Centromeric localization requires the presence of BUB1 and AUKRB.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Sgo2 antibody
    • Sgol2 antibody
    • SGOL2_HUMAN antibody
    • Shugoshin-2 antibody
    • Shugoshin-like 2 antibody
    • Tripin antibody
    see all

图片

  • Anti-SGOL2 antibody (ab122258) + HEL human cell line lysate

    Predicted band size : 145 kDa
  • ab122258, at 1/100, staining SGOL2 in paraffin embedded Human testis tissue by Immunohistochemistry.
  • ab122258, at 4 µg/ml, staining SGOL2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green stain).

文献

ab122258 has not yet been referenced specifically in any publications.

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