Customer reviews and FAQs

概述

  • 产品名称
    Anti-SGLT2抗体
    参阅全部 SGLT2 一抗
  • 描述
    兔多克隆抗体to SGLT2
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SGLT2.

    参阅Abcam的专有抗源政策

  • 阳性对照
    • This antibody gave a positive signal in Human Kidney Tissue (Immunohistochemistry).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 73 kDa. PubMed: 25268124
ICC/IF Use at an assay dependent concentration. PubMed: 25268124

靶标

  • 功能
    Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
    Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • 疾病相关
    Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
  • 序列相似性
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Low affinity sodium glucose cotransporter antibody
    • Low affinity sodium-glucose cotransporter antibody
    • Na(+) glucose cotransporter 2 antibody
    • Na(+)/glucose cotransporter 2 antibody
    • OTTHUMP00000163298 antibody
    • SC5A2_HUMAN antibody
    • SGLT 2 antibody
    • SLC5A 2 antibody
    • SLC5A2 antibody
    • Sodium glucose cotransporter 2 antibody
    • Sodium/glucose cotransporter 2 antibody
    • Solute carrier family 5 (sodium glucose cotransporter) member 2 antibody
    • Solute carrier family 5 (sodium/glucose transporter), member 2 antibody
    • Solute carrier family 5 member 2 antibody
    see all

Anti-SGLT2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGLT2 antibody (ab85626)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGLT2 antibody (ab85626)
    IHC image of SGLT2 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85626, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody (ab85626)
    Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody (ab85626)This image is courtesy of an anonymous Abreview.

    Immunohistochemical analysis of rat kidney frozen sections, labeling SGLT2 with ab85626. Sections were paraformaldehyde fixed, permeabilzed in 0.1% Triton-X, and blocked with 1% BSA for 30 minutes at 20°C. Immunostaining with anti-SGLT2 diluted 1/200 (1% BSA and 1% normal donkey serum in PBS) for 16 hours at 4°C.

    See Abreview

Anti-SGLT2 antibody (ab85626)参考文献

This product has been referenced in:
  • Blanco AM  et al. Ghrelin Facilitates GLUT2-, SGLT1- and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus). Sci Rep 7:45024 (2017). Read more (PubMed: 28338019) »
  • Jiang M  et al. Sodium-Glucose Transporter-2 (SGLT2; SLC5A2) Enhances Cellular Uptake of Aminoglycosides. PLoS One 9:e108941 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 25268124) »

See all 2 Publications for this product

发表研究结果有使用 ab85626？请让我们知道，以便我们可以引用本数据表中的参考文章。

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提交 ab85626 的 abreview 提交 ab85626 的问题

8 Abreviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SGLT2 antibody

 Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung tumors)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TrisHcl-EDTA pH 8.0
Permeabilization
No
Specification
lung tumors
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
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Username

Claudio Scafoglio

Verified customer

提交于 Jul 27 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SGLT2 antibody

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (vessel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
vessel
Blocking step
dako antibody diluent with background reducing components as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Fixative
Formaldehyde
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Miss. Seulgee Lee

Verified customer

提交于 Feb 21 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SGLT2 antibody

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer(pH6)
Permeabilization
No
Specification
Kidney
Blocking step
(agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
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Username

Miss. Hsiu-Yun Huang

Verified customer

提交于 Mar 04 2016

Immunohistochemistry (Frozen sections) abreview for Anti-SGLT2 antibody

 Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidney)
Permeabilization
Yes - 0.1% Triton-X
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Sep 08 2015

I would like to try the anti-SGLT2 antibody ab85626, but I need a peptide inhibitor to check the specificity. I was wondering if you sell the peptide too or not.

We do provide the immunogen peptide for ab85626. Our SGLT2 peptide (ab101414) may be found at the following location: http://www.abcam.com/sglt2-peptide-ab101414.html


Thank you very much for your reply and offer! I would like to proceed and test ab85625 in Mouse, and will wait for your discount code.
Best

DISCOUNT CODE: *******
Expiration date: DD MM YYYY
I am very pleased to hear you would like to accept our offer and test ab85626 in Mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please...

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Product code: 85626
Inquiry: We would like to test Anti-SGLT2 antibody (ab85626) in murine kidney. Is het possible to buy a small amount of Ab to test it in murine samples (on your website it is only tested in human, and predicted to work in mouse)...

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Thank you very much for your interest in ab85625.
To our knowledge, ab85626 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab85625 now, test it in Mouse and submit feedback to us in the form of an ...

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Would like to test this antibody in guinea pig or mouse.

DISCOUNT CODE: *** Expiration date: April 20, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab85626 in guinea pig or mouse and include this code in the “Additional C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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