- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-SGLT2抗体
参阅全部 SGLT2 一抗 -
描述兔多克隆抗体to SGLT2
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经测试应用适用于: IHC-P, WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SGLT2.
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阳性对照
- This antibody gave a positive signal in Human Kidney Tissue (Immunohistochemistry).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab85626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 73 kDa. PubMed: 25268124 | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 25268124 |
靶标
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功能Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
疾病相关Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
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序列相似性Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 399680 Cow
- Entrez Gene: 492301 Dog
- Entrez Gene: 6524 Human
- Entrez Gene: 246787 Mouse
- Entrez Gene: 64522 Rat
- Omim: 182381 Human
- SwissProt: P31639 Human
- SwissProt: Q923I7 Mouse
see all -
别名
- Low affinity sodium glucose cotransporter antibody
- Low affinity sodium-glucose cotransporter antibody
- Na(+) glucose cotransporter 2 antibody
see all
Anti-SGLT2 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGLT2 antibody (ab85626)IHC image of SGLT2 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85626, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX -
Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody (ab85626)This image is courtesy of an anonymous Abreview.Immunohistochemical analysis of rat kidney frozen sections, labeling SGLT2 with ab85626. Sections were paraformaldehyde fixed, permeabilzed in 0.1% Triton-X, and blocked with 1% BSA for 30 minutes at 20°C. Immunostaining with anti-SGLT2 diluted 1/200 (1% BSA and 1% normal donkey serum in PBS) for 16 hours at 4°C.
实验方案
Anti-SGLT2 antibody (ab85626)参考文献
This product has been referenced in:
- Jiang M et al. Sodium-Glucose Transporter-2 (SGLT2; SLC5A2) Enhances Cellular Uptake of Aminoglycosides. PLoS One 9:e108941 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 25268124) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"