Anti-SGLT2抗体(ab58298)

概述

性能

应用

Our Abpromise guarantee covers the use of ab58298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
    Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • 疾病相关Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
  • 序列相似性Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Low affinity sodium glucose cotransporter antibody
    • Low affinity sodium-glucose cotransporter antibody
    • Na(+) glucose cotransporter 2 antibody
    • Na(+)/glucose cotransporter 2 antibody
    • OTTHUMP00000163298 antibody
    • SC5A2_HUMAN antibody
    • SGLT 2 antibody
    • SLC5A 2 antibody
    • SLC5A2 antibody
    • Sodium glucose cotransporter 2 antibody
    • Sodium/glucose cotransporter 2 antibody
    • Solute carrier family 5 (sodium glucose cotransporter) member 2 antibody
    • Solute carrier family 5 (sodium/glucose transporter), member 2 antibody
    • Solute carrier family 5 member 2 antibody
    see all

Anti-SGLT2 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa
  • Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-SGLT2 antibody (ab58298)参考文献

ab58298 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
Sample Human Cell (Human Renal Proximal Tubular Endothelial Cells)
Specification Human Renal Proximal Tubular Endothelial Cells
Permeabilization Yes - triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 04 2015

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (CHO-K1 cell line)
Loading amount 30 µg
Specification CHO-K1 cell line
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jagadeesh Daler

Verified customer

提交于 Nov 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"