Anti-SGLT1抗体(ab14685)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SGLT1抗体
参阅全部 SGLT1 一抗 -
描述
兔多克隆抗体to SGLT1 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 75% homology with SGLT5. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SGLT1 aa 600-700.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 2 µg/ml.
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说明 |
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IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
组织特异性
Expressed mainly in intestine and kidney. -
疾病相关
Congenital glucose/galactose malabsorption -
序列相似性
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
翻译后修饰
N-glycosylation is not necessary for the cotransporter function. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6523 Human
- Omim: 182380 Human
- SwissProt: P13866 Human
- Unigene: 1964 Human
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别名
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
图片
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ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab14685 被引用在 22 文献中.
- Du J et al. The expression and survival significance of sodium glucose transporters in pancreatic cancer. BMC Cancer 22:116 (2022). PubMed: 35090421
- Park SH et al. Angiotensin II-induced upregulation of SGLT1 and 2 contributes to human microparticle-stimulated endothelial senescence and dysfunction: protective effect of gliflozins. Cardiovasc Diabetol 20:65 (2021). PubMed: 33726768
- Ranea-Robles P et al. Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice. Kidney360 2:1441-1454 (2021). PubMed: 34651140
- Chiba Y et al. Sodium/glucose cotransporter 2 is expressed in choroid plexus epithelial cells and ependymal cells in human and mouse brains. Neuropathology N/A:N/A (2020). PubMed: 32488949
- Chiba Y et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 33008107