概述
- 产品名称Anti-SGLT1抗体
参阅全部 SGLT1 一抗 - 描述兔多克隆抗体to SGLT1
- 经测试应用适用于: IHC-Pmore details
- 种属反应性与反应: Mouse
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Mouse SGLT1.
- 阳性对照
- This antibody gave a positive signal in Mouse Liver formalin fixed paraffin embedded tissue section.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab105755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
- 功能Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
- 组织特异性Expressed mainly in intestine and kidney.
- 疾病相关Congenital glucose/galactose malabsorption
- 序列相似性Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
- 翻译后修饰N-glycosylation is not necessary for the cotransporter function.
- 细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 20537 Mouse
- SwissProt: Q8C3K6 Mouse
- Unigene: 25237 Mouse
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别名
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
Anti-SGLT1 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody (ab105755)IHC image of SGLT1 staining in Mouse Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105755, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-SGLT1 antibody (ab105755)参考文献
ab105755 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"