Anti-SGK1 (phospho S422)抗体(ab55281)

概述

  • 产品名称
    Anti-SGK1 (phospho S422)抗体
    参阅全部 SGK1 一抗
  • 描述
    兔多克隆抗体to SGK1 (phospho S422)
  • 特异性
    ab55281 detects endogenous levels of SGK1 only when phosphorylated at serine 422.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of serine 422 (G-F-SP-Y-A).

  • 阳性对照
    • WB: Insulin treated HeLa cell extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab55281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 49 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能
    Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
  • 组织特异性
    Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 结构域
    Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
  • 翻译后修饰
    Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
    Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
  • 细胞定位
    Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • OTTHUMP00000017247 antibody
    • Serine/threonine protein kinase SGK antibody
    • Serine/threonine protein kinase Sgk1 antibody
    • Serine/threonine-protein kinase Sgk1 antibody
    • Serum and glucocorticoid regulated kinase antibody
    • Serum/glucocorticoid regulated kinase 1 antibody
    • Serum/glucocorticoid regulated kinase antibody
    • Serum/glucocorticoid-regulated kinase 1 antibody
    • SGK 1 antibody
    • SGK antibody
    • SGK1 antibody
    • Sgk1 variant i3 antibody
    • SGK1_HUMAN antibody
    see all

图片

  • All lanes : Anti-SGK1 (phospho S422) antibody (ab55281) at 1/500 dilution

    Lane 1 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes) plus immunizing phosphopeptide
    Lane 2 : Extracts from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with Insulin (0.01 U/ml, 15 minutes)

    Developed using the ECL technique

    Predicted band size : 49 kDa
  • Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using ab55281 at a 1/50 diluton.

    Left: No phosphopeptide.

    Right: Sample treated with immunizing phosphopeptide.

    Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using 1/50 ab55281. Left: no phosphopeptide. Right: sample treated with immunising phosphopeptide.
  • ICC/IF image of ab55281 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 4% PFA for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55281, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

文献

This product has been referenced in:
  • Fu L  et al. Lactic acid bacteria-specific induction of CD4(+)Foxp3(+)T cells ameliorates shrimp tropomyosin-induced allergic response in mice via suppression of mTOR signaling. Sci Rep 7:1987 (2017). WB ; Mouse . Read more (PubMed: 28512288) »
  • Venkatesh A  et al. Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice. J Clin Invest 125:1446-58 (2015). Read more (PubMed: 25798619) »

See all 8 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Joby Westmoreland

Verified customer

提交于 Mar 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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