Anti-SFXN1抗体(ab86552)

概述

  • 产品名称Anti-SFXN1抗体
    参阅全部 SFXN1 一抗
  • 描述
    兔多克隆抗体to SFXN1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (SGELPPNINI KEPRWDQSTF IGRANHFFTV TDPRNILLTN EQLESARKIV) of human SFXN1 (NP_073591).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab86552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-SFXN1 antibody 图像

  • Anti-SFXN1 antibody (ab86552) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : ~32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~57 kDa. We are unsure as to the identity of these extra bands.

Anti-SFXN1 antibody (ab86552)参考文献

ab86552 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (12%)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"